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sbcD [2018-10-30 18:31:44]
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sbcD [2018-10-30 18:31:44]

exonuclease SbcD homolog
locus
BSU10640
pI
5.00
mw
43.25 kDa
protein length
391 aa Sequence Blast
gene length
1173 bp Sequence Blast
function
unknown
product
exonuclease SbcD homolog
essential
no
synonyms
yixA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

    • Gene

    Coordinates
    1,143,577 → 1,144,752

    The protein

    Protein family

  • sbcD family (according to Swiss-Prot)

    • Structure

  • [PDB|4LTY] (from E. coli, corresponds to aa 1 - 314 of SbcD, 30% identity) [pubmed|24531464]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operons

    genes
    [gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-[gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|4D89BFFB1073407A7A1762AFD18B49F3414D6787|sbcD]-[gene|ED825D5B3290BA8F9C92477B1FCC433ADB3C8449|sbcC]-[gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7746142], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

    • regulation

  • positive control by [protein|search|ComK] [Pubmed|7746142]
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    Biological materials

    Mutant

  • GP894 (''sbcCD''::''kan''), available in [SW|Stülke] lab
  • BKE10640 (Δ[gene|4D89BFFB1073407A7A1762AFD18B49F3414D6787|sbcD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGCTTTCACCTCTCTT, downstream forward: _UP4_GGCGTTGAAGAGGAGGATGC
  • BKK10640 (Δ[gene|4D89BFFB1073407A7A1762AFD18B49F3414D6787|sbcD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10640 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATGCTTTCACCTCTCTT, downstream forward: _UP4_GGCGTTGAAGAGGAGGATGC

    • References

  • 8493111,11948146,22383849,16479537,24531464